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US 8,771,647 B2Granted
Human photoaged skin model
AssigneeKao Corporation
InventorsAkira Hachiya, Tsutomu Fujimura
Life of this patent
Estimating the term…
Abstract
The present invention provides a human photoaged skin model and an animal model, and a method of making the same, for evaluating cosmetics and similar products in terms of their anti-aging or rejuvenating effect.
Claims26
A method for producing a human photoaged skin model, comprising:
- irradiating a skin area of an immunodeficient mouse;
- and
- discontinuing irradiation when the skin area is in a condition suitable for use as a human photoaged skin model;
- wherein:
- the skin area comprises human skin transplanted to the immunodeficient mouse;
- irradiating the skin area comprises irradiating for a period of from six to eight consecutive weeks, during which:
- the skin area is irradiated with 40 to 50 mJ/cm2 of UV-B radiation during a first week of the period;
- the skin area is irradiated with 50 to 100 mJ/cm2 of UV-B radiation during a second week of the period;
- the skin area is irradiated with 60 to 100 mJ/cm2 of UV-B radiation during a third week, a fourth week, a fifth week, and a sixth week of the period;
- and
- the skin area is optionally irradiated with 60 to 100 mJ/cm2 of UV-B radiation during an optional seventh week and an optional eighth week of the period;
- irradiating the skin area comprises irradiating with a total dose of UV-B radiation of from approximately 1.65 J/cm2 to approximately 2.85 J/cm2;
- and
- the skin area is in the condition suitable for use as a human photoaged skin model when the skin area has at least one condition selected from the group consisting of wrinkles, sagginess, age spots, freckles, lowered elasticity, increased darkening, increased yellowing, and reduced keratinous layer water content.
11 more elements
A method for producing a human photoaged skin model, comprising:
- irradiating a skin area of an immunodeficient mouse;
- and
- discontinuing irradiation when the skin area is in a condition suitable for use as a human photoaged skin model;
- wherein:
- the skin area comprises human skin transplanted to the immunodeficient mouse;
- irradiating the skin area comprises irradiating for a period of from six to eight consecutive weeks, during which:
- the skin area is irradiated with 80 to 90 mJ/cm2 of UV-B radiation during a first week of the period;
- the skin area is irradiated with 90 to 100 mJ/cm2 of UV-B radiation during a second week of the period;
- the skin area is irradiated with 100 mJ/cm2 of UV-B radiation during a third week, a fourth week, a fifth week, and a sixth week of the period;
- and
- the skin area is optionally irradiated with 100 mJ/cm2 of UV-B radiation during an optional seventh week and an optional eighth week of the period;
- irradiating the skin area comprises irradiating with a total dose of UV-B radiation of from approximately 1.65 J/cm2 to approximately 2.85 J/cm2;
- and
- the skin area is in the condition suitable for use as a human photoaged skin model when the skin area has at least one condition selected from the group consisting of wrinkles, sagginess, age spots, freckles, lowered elasticity, increased darkening, increased yellowing, and reduced keratinous layer water content.
11 more elements
Dependencies are inferred from the claim text.