Method for the producing alpha-1, 6-branched alpha-1, 4-glucans from sucrose
AssigneesBayer BioScience N.V., Max-Planck-Gesellschaft zur Foderung der Wissenchaften E.V.
InventorMartin Quanz
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Abstract
Nucleic acid molecules which encode a branching enzyme from a bacterium of the genus Neisseria, vectors, host cell, plant cells and plants containing said nucleic acid molecules as well as starch obtainable from the plants described are described. Furthermore, an in-vitro method for producing α-1,6-branched α-1,4-glucans on the basis of sucrose and a combination of enzymes of an amylosucrase and a branching enzyme as well as the α-1,6-branched α-1,4-glucans obtainable by said method are described.
Claims4
Starch prepared by the process that comprises extracting starch from transgenic plants or parts thereof that comprise transgenic plant cell containing a nucleic acid molecule linked to regulatory sequences guaranteeing the transcription in plant cells, wherein said nucleic acid molecule encodes a branching enzyme from a bacterium of the genus Neisseria selected from the group consisting of
- (a) nucleic acid molecules encoding a protein which comprises the amino acid sequence depicted in SEQ ID NO. 2;
- (b) nucleic acid molecules comprising the coding region depicted in SEQ ID NO. 1;
- (c) nucleic acid molecules encoding a protein which comprises the amino acid sequence encoded by the insert in plasmid DSM 12425;
- (d) nucleic acid molecules comprising the coding region for a branching enzyme, which is contained in the insert of the plasmid DSM 12425;
- and
- (e) nucleic acid molecules encoding a protein the sequence of which has, in the first 100 amino acids, a homology of at least 65% to the amino acid sequence depicted in SEQ ID NO. 2;
- wherein said starch has a phosphate content that is at last 20% reduced as compared to starch from a corresponding wild type plant.
Dependencies are inferred from the claim text.