Methods and compositions for liposomal formulation of antigens and uses thereof
AssigneesThe Regents of the University of California, Molecular Express, Inc.
InventorsGary Fujii, Francis C. Szoka, Jr., Douglas S. Watson
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Abstract
The present invention relates to liposomal vaccine compositions, methods for the manufacture thereof, and methods for the use thereof to stimulate an immune response in an animal. These compositions comprise dimyristoylphosphatidylcholine (“DMPC”); either dimyristoylphosphatidylglycerol (“DMPG”) or dimyristoyltrimethylammonium propane (“DMTAP”) or both DMPC and DMTAP; and at least one sterol derivative providing a covalent anchor for one or more immunogenic polypeptide(s) or carbohydrate(s).
Claims21
A composition comprising one or more immunogenic polypeptides of interest, the composition comprising:
- a) an aqueous vehicle;
- b) liposomes comprising
- (i) dimyristoylphosphatidylcholine (“DMPC”),
- (ii) one or more lipids selected from the group consisting of dimyristoylphosphatidylglycerol (“DMPG”), and dimyristoyltrimethylammonium propane (“DMTAP”), and
- (iii) at least one sterol derivative,
- at relative percentages of 50%-98% (i):
- 1%-25% (ii):
- 1%-25% (iii);
- and
- c) said one or more immunogenic polypeptide(s) covalently linked to between 1% and 100% of said at least one sterol derivative.
Dependencies are inferred from the claim text.