Enzyme method of extracting oils and proteins from vegetable matter in an aqueous medium

An enzyme method for extraction in an aqueous medium, oils, proteins and fermentable sugars from vegetable material, comprising the following steps:

• a) adding water to the vegetable material having a suitable particle size,
• b) adding an enzyme mixture containing at least one cellulose, at least one hemicellulose, and at least one pectinase, the ratio between the pectinase activity and the cellulose activity being at least 0.14, the ratio between the pectinase activity and the hemicellulose activity being between 1×10−2 and 0.5, and the pectinase activity being less than 120 μmol/min/mL,
• c) incubating the vegetable material and the enzyme mixture with stirring in order to release in the reaction medium oils, proteins and fermentable sugars, for a duration depending on the sought yields,
• d) separating the reaction medium in order to obtain free oil, an aqueous phase containing proteins and fermentable sugars, and a solid phase,
• e) optionally separating an emulsion of the free oil or of the aqueous phase, and recycling the emulsion in the reaction medium,
• f) separating the proteins and fermentable sugars from the aqueous phase.