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AssigneeMascoma Corporation
InventorsElena E. Brevnova, Vineet Rajgarhia, Mark Mellon, Anne Warner, John McBride, Chhayal Gandhi, Erin Wiswall
Estimating the term…
The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
An isolated polynucleotide comprising a nucleic acid which encodes the mature endoglucanase of SEQ ID NO:36, wherein said nucleic acid is codon-optimized for expression in a yeast strain wherein at least one nucleotide within a sequence of 4, 5, 6, 7, 8, 9, or 10 consecutive A, T, C or G nucleotides is replaced with a different nucleotide, wherein the nucleotide replacement does not alter the amino acid sequence encoded by the polynucleotide and wherein the nucleotide replacement creates a codon that is the second most frequently used codon to encode an amino acid in the yeast strain.
An isolated polynucleotide comprising a nucleic acid which encodes the mature endoglucanase of SEQ ID NO:36, wherein said nucleic acid is codon-optimized for expression in a s east strain, wherein at least one restriction enzyme site within the polynucleotide is removed by replacing at least one nucleotide within the restriction enzyme site with a different nucleotide, wherein the nucleotide replacement does not alter the amino acid sequence encoded by the polynucleotide and wherein the nucleotide replacement creates a codon that is the second most frequently used codon to encode an amino acid in the yeast strain.
An isolated polynucleotide comprising a nucleic acid which encodes the mature endoglucanase of SEQ ID NO:36, wherein said nucleic acid is codon-optimized for expression in a yeast strain, wherein one or more direct repeats, inverted repeats and mirror repeats with lengths of 10 bases or longer within said polynucleotide is altered by replacing at least one nucleotide within the repeat with a different nucleotide, wherein the nucleotide replacement does not alter the amino acid sequence encoded by the polynucleotide and wherein the nucleotide replacement creates a codon that is the second most frequently used codon to encode an amino acid in the yeast strain.
An isolated polynucleotide comprising a nucleic acid, wherein the nucleic acid comprises the a nucleic acid coding sequence of SEQ ID NO:16.
A host cell comprising a polynucleotide encoding the mature endoglucanase of SEQ ID NO:36 wherein the host cell is a yeast cell, wherein the polynucleotide is codon-optimized for expression in yeast and wherein the endoglucanase is expressed, wherein the host cell is co-cultured with one or more additional host cells, wherein each of the one or more additional host cells expresses one or more endoglucanases, cellobiohydrolases and/or β-glucosidases.
A host cell comprising a polynucleotide encoding the mature endoglucanase of SEQ ID NO:
Dependencies are inferred from the claim text.