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US 8,570,500 B2Granted
Detector arrangement for a flow cytometry system
AssigneeStratedigm, Inc.
InventorsShervin Javadi, Arjuna Karunaratne
Life of this patent
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Abstract
Systems and methods for regarding a cytometry system are disclosed. The system can include a plurality lasers which are spatially separated from each other. Each laser can be assigned to a single detector. The single detector can process multiple events from each laser by digital switching of signal processing circuitry. Additional detectors can be assigned to receive light in a similar manner.
Claims22
A cytometry system comprising:
- a computing system;
- a plurality of laser sources controlled by the computing system to emit laser light, each laser source being spatially separated along a flow stream path;
- a light condenser for collecting a plurality of light pulses that are separated by time of flight intervals, each light pulse being derived from the spatially separated laser light intersecting material flowing in the flow stream path;
- and
- a first detector configured to receive the plurality of light pulses from the light condenser, the first detector being coupled to sampling circuitry,
- wherein the computing system is configured to operate the sampling circuitry to process each light pulse according to the time of flight intervals,
- wherein the computing system is configured to turn the sampling circuitry OFF during the time of flight intervals by actively discharging residual energy of one or more energy storage filter elements of the sampling circuitry,
- wherein before each time of flight interval the residual energy is caused by filtering a prior light pulse, and wherein the residual energy caused by filtering the prior light pulse is actively discharged from the one or more energy storage filter element to prevent interference for processing a subsequent pulse by the sampling circuitry.
5 more elements
A method for operating a cytometry system, the method comprising:
- receiving a first light pulse derived from a first laser interacting with a material;
- triggering a first ON signal over a duration of a first predetermined sampling interval;
- deriving pulse information from the first light pulse signal during the first ON signal via signal processing circuitry;
- triggering an OFF signal to stop the signal processing circuitry from deriving pulse information from the first light pulse signal, the OFF signal corresponding to a time of flight interval of the material between the first laser and a spatially separated second laser;
- receiving a second light pulse signal derived from the second laser interacting with the material;
- triggering a second ON signal over a duration of a second predetermined sampling interval;
- and
- deriving pulse information from the second light pulse signal during the second ON signal,
- wherein residual energy of at least one energy storing filter element of the signal processing circuitry is actively discharged during the OFF signal,
- wherein before each time of flight interval the residual energy is caused by filtering the first light pulse, and wherein the residual energy caused by filtering the first light pulse is actively discharged from the energy storage filter element to prevent interference for processing the second light pulse by the sampling circuitry.
7 more elements
Dependencies are inferred from the claim text.