Secreted insecticidal protein and gene compositions from Bacillus thuringiensis and uses therefor

A method for expressing a lepidopteran-active toxin protein in a plant, comprising the steps of:

• (a) inserting into the genome of a plant cell a nucleic acid sequence comprising in the 5′ to 3′ direction an operably linked recombinant, double-stranded DNA molecule, wherein the recombinant, double-stranded DNA molecule comprises:
• (i) a promoter that functions in the plant cell;
• (ii) a nucleotide sequence encoding the insecticidal amino acid sequence as set forth in SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:30, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or SEQ ID NO:20;
• and
• (iii) a 3′ non-translated nucleotide sequence that functions in the cells of the plant to cause termination of transcription;
• (b) obtaining a transformed plant cell containing the nucleic acid sequence of step (a);
• and
• (d) generating from said transformed plant cell a plant that expresses the lepidopteran-active toxin protein in the transformed plant.