Loading patent…
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsXiaohong Wang, Shunwen Lu
Estimating the term…
Plant CLAVATA3/ESR-related (CLE) peptides have diverse roles in plant growth and development. We have isolated and characterized the function of five new CLE genes from the potato cyst nematode Globodera rostochiensis. Unlike typical plant CLEs that contain a single CLE motif, four of the five Gr-CLE genes encode CLE proteins with multiple CLE motifs. These Gr-CLEs were found to be specifically expressed within the dorsal esophageal gland cell of nematode parasitic stages, suggesting a role for their encoded proteins in plant parasitism. Overexpression of Gr-CLEs in Arabidopsis mimicked overexpression of plant CLEs and Gr-CLE proteins could rescue the Arabidopsis clv3-2 mutant phenotype when expressed within meristems. A short root phenotype was observed when synthetic GrCLE peptides were exogenously applied to roots of Arabidopsis or potato similar to the overexpression of Gr-CLEs in Arabidopsis and potato hairy roots. These results reveal that G. rostochiensis CLEs with either single or multiple CLE motifs function similarly to plant CLEs and that CLE signaling components are conserved in both Arabidopsis and potato roots. Transgenic potato hairy roots expressing Gr-CLE-1 or Gr-CLE-4 dsRNA were generated. There was an approximately 50% reduction in the average number of cysts per root in the Gr-CLE-1 or Gr-CLE-4 dsRNA transgenic lines when compared with the infected control lines, indicating that silencing nematode CLE genes through host-derived RNAi may generate novel resistance against potato cyst nematodes in transgenic potatoes.
An isolated polynucleotide that produces a double stranded ribonucleotide hairpin, said polynucleotide consisting of a fragment of at least 21 contiguous nucleotides of a nucleic acid which encodes SEQ ID NO:23, and a reverse complement of said fragment;
An isolated double stranded ribonucleotide molecule, defined as produced by preparing a recombinant polynucleotide molecule comprising a first and a second polynucleotide sequence, wherein the first polynucleotide sequence consists of an isolated polynucleotide molecule which encodes SEQ ID NO:23, wherein the second polynucleotide sequence is substantially the reverse complement of the first polynucleotide sequence such that the first and the second polynucleotide sequences hybridize when the recombinant polynucleotide molecule is transcribed into a ribonucleic acid molecule that forms the double stranded ribonucleotide molecule, wherein the double stranded ribonucleotide molecule inhibits the growth of a G. rostochiensis nematode.
A plant transformation vector comprising a polynucleotide molecule that produces a double stranded ribonucleotide molecule, said polynucleotide molecule consisting of a fragment of at least 21 contiguous nucleotides of a nucleic acid which encodes SEQ ID NO:23, and a reverse complement of said fragment;
A method for controlling a G. rostochiensis parasitic nematode population, comprising:
Dependencies are inferred from the claim text.