Method for preparing oligonucleotide
AssigneeSuzhou Ribo Life Science Co., Ltd.
InventorsZhen Xi, Jinyu Huang, Junbin Zhang
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Abstract
A method for preparing oligonucleotide comprising reacting the compound of Formula (1) with the compound of Formula (2) in a liquid reaction medium under the condition of condensation reaction to obtain the compound of formula (3) is provided. 1-(2-mesitylenesulfonyl)-3-nitro-1H-1,2,4-triazole (MSNT) is applied as condensing agent. Oligonucleotides synthesized in the liquid reaction medium could be obtained on a large scale.
Claims11
A method for preparing an oligonucleotide, comprising reacting the compound represented by Formula (1) with the compound represented by Formula (2) in a liquid reaction medium under the condition of condensation reaction to obtain the compound represented by Formula (3), wherein 1-(2-mesitylenesulfonyl)-3-nitro-1H-1,2,4-triazole (MSNT) is applied as condensing agent,
- and wherein:
- R1 represents 4,4′-dimethoxytriphenylmethyl, or a covalently attached 5′-substituent derived from RNA or from DNA;
- R2 and R3 independently represent a sterically hindered trisubstituted silyl protective group;
- R4 represents a halogen atom;
- A+ represents a tri-alkyl ammonium ion;
- B1 and B2 independently represent 9-guaninyl substituted with N-acyl, 9-adeninyl substituted with N-acyl, 1 cytosinyl substituted with N-acyl, 1-thyminyl or 1-uracilyl;
- and with the proviso that in Formula (1) or (3), —CH2—N3 is at an ortho-position.
Dependencies are inferred from the claim text.