US 8,569,464 B2 · Purification of proteins

Abstract

The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.

Claims33

Dependent claims

A method for purifying a biomolecule from a mixture containing soluble and insoluble impurities, comprising:

a. providing the mixture at a set of conditions, b. adding one or more polyvinyl pyridine polymers or copolymers to said mixture, soluble in said mixture under the set of conditions and capable of reversibly and selectively binding to the biomolecule, and capable of irreversibly binding to said insoluble impurities, c. mixing the one or more solubilized polymers throughout the mixture;
d. precipitating the one or more polymers, insoluble impurities and said biomolecule out of solution by changing the set of conditions in the mixture;
e. separating the precipitate from the mixture, and f. selectively eluting the biomolecule from the precipitate.

Dependencies are inferred from the claim text.

Purification of proteins

Life of this patent

Purification of proteins

Life of this patent

Abstract

Claims33