Radioimmunoassay with a 96-welled micro-plate

A method for radioimmunoassay for measuring concentrations of a target material in samples including the steps of:

• providing a multi-welled micro-plate 21 that can be dismantled;
• executing radioimmunoassay including the steps of:
• filling an antibody 31, specific for the target material, in the wells 22 of the multi-welled micro-plate 21 so that the antibody 31 is coated on an internal side of each of the wells 22 of the multi-welled micro-plate 21;
• washing away an excessive portion of the antibody 31 from the wells 22;
• coating an blocking reagent 32 on a portion of the surface of the multi-welled micro-plate 21 that is not covered by the antibody 31;
• removing an excessive portion of the blocking reagent 32;
• filling samples 33 of a target material in the wells 22;
• oscillating the multi-welled micro-plate 21 for a predetermined period of time;
• washing away an excessive portion of the samples 33 is by washing buffer for several times;
• labeling an antibody 34 specific for the target material with iodine-125 and filling it in the wells 22;
• oscillating the multi-welled micro-plate 21 for another predetermined period of time;
• washing away an excessive portion of the antibody 34 labeled with the iodine-125 by washing buffer for several times;
• and
• inverting and locating the multi-welled micro-plate 21 on a tower of tissue paper so that all liquid leaves the wells 22;
• and
• executing a gamma counting including the steps of:
• dismantling the multi-welled micro-plate 21 into separate wells 22;
• casting each of the wells 22 in a test tube 23;
• and
• providing a gamma counter 24 is used for the gamma counting in the test tubes 23, thus measuring the concentrations of the target material in the samples 33.