US 8,569,064 B2Granted

Manipulation of flavonoid biosynthesis in plants

AssigneesAgriculture Victoria Services PTY LTD, AgResearch Limited

InventorsGerman Spangenberg, Timothy Ivor Sawbridge, Eng-Kok Ong, Michael Emmerling

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Abstract

The present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3′,5′ hydrolase (F3′5′H), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof.

Claims8

Dependent claims

A substantially purified or isolated nucleic acid, said nucleic acid being selected from the group consisting of:

(a) a nucleotide sequence selected from the group consisting of SEQ ID Nos:
185 and 187 to 193;
(b) a nucleotide sequence comprising at least bases 1 to 311 of Seq ID No. 193,
(c) a nucleotide sequence which is the full length complement to a nucleotide sequence selected from the group consisting of SEQ ID Nos:
185 and 187 to 193;
and
(d) a probe nucleotide sequence, wherein the entire probe nucleotide sequence is the same as or complementary to a portion of a target sequence, said target sequence having a sequence as defined in paragraph (a), wherein the probe nucleotide sequence has a length sufficient for sequence specific hybridization with the target sequence.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 12/844,615Filed 27 Jul 2010 · division
Priority: AU PR 81135 Oct 2001
Published: US 2011/0055974 A13 Mar 2011
Granted: 29 Oct 2013
Term ends: Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 9/93
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: ligases
A61P 25/28
Drugs for disorders of the nervous system: for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents,…
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12N 15/825
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving pigment biosynthesis

Family

PCT/AU02/01345Parent of this division · filed 4 Oct 2002
US 7,767,416 B2Parent of this division · filed 13 Aug 2010
US 2011/0055974 A1Pre-grant publication · published 3 Mar 2011
Application10/491,823Parent of this division
US 2005/0069884 A1Published application
US 8,569,064 B2This patent

From the US filing history. Foreign family members aren’t listed.

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