Method of producing a population of cells

A method of increasing the number of haematopoietic cells in a patient, the method comprising:

• (a) providing a population of cells comprising haematopoietic progenitor cells;
• (b) culturing the population of cells in an animal cell culture medium comprising one or more cytokines that differentiate said progenitor cells into lineage committed haematopoietic progenitor and/or mature haematopoietic cells, under static conditions until the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and progeny thereof under static conditions, and then agitating the culture medium thereafter, to produce a population of lineage committed haematopoietic progenitor or mature haematopoietic cells other than cells of the neutrophil lineage;
• and
• (c) administering to the patient the population of lineage committed haematopoietic progenitor or mature haematopoietic cells other than cells of the neutrophil lineage obtained in step (b) such that the number of haematopoietic cells in the patient is increased.