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US 8,569,059 B2Granted
Method of identifying CD4+ CD25+ T-cells activated to an antigen which express CD8
AssigneeNewSouth Innovations Pty Limited
InventorsBruce Milne Hall, Suzanne J. Hodgkinson
Life of this patent
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Abstract
The invention relates to a method of identifying CD4+ CD25+ T cells activated to an antigen in a population of T cells, comprising identifying CD4+ CD25+ T cells which express CD8. The invention also relates to a method of isolating CD4+ CD25+ T cells activated to an antigen comprising isolating CD4+ CD25+ T cells which express CD8. The invention also relates to a method of increasing tolerance to an antigen in a subject comprising administering CD4+ CD25+ T cells which express CD8.
Claims11
A method of preparing a population of activated CD4+CD25+CD8+ T cells comprising:
- (a) providing a population of isolated CD4+CD25+ T cells;
- (b) contacting the population of isolated CD4+CD25+ T cells with an alloantigenic antigen presenting cell, in the presence of a factor selected from the group consisting of IL-2 and IL-4, for a sufficient time to permit proliferation of the CD4+,CD25+ T cells;
- and
- (c) isolating CD4+CD25+CD8+ T cells from the proliferated CD4+CD25+ T cells.
1 more element
Dependencies are inferred from the claim text.