DNase expression in recombinant host cells
AssigneeNovozymes A/S
InventorsMichael Dolberg Rasmussen, Jon Martin Persson
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Abstract
The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.
Claims20
A Bacillus cell transformed with a first expression vector comprising a nucleic acid encoding at least one enzyme of interest and a second expression vector comprising a nucleic acid encoding at least one nuclease, wherein the Bacillus cell is able to secrete the enzyme(s) of interest and nuclease(s) into a fermentation broth resulting in reduced DNA in the fermentation broth compared to an otherwise isogenic Bacillus cell without the vector that encodes at least one nuclease.
The method in accordance with 9, wherein the enzyme is an oxidoreductase.
Dependencies are inferred from the claim text.