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US 8,569,007 B2Granted
Method for reducing the appearance of false positive bands in SDS-page analysis of proteolytic digestion of a sample
AssigneeMerck Sharp & Dohme Corp.
InventorsKimberly Margaret Louise May, Susan V. Cannon-Carlson, Brittany Charlotte Larkin, Collette Marie Cutler
Life of this patent
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Abstract
The present invention includes a method for reducing the appearance of false positive bands in SDS-PAGE analysis of proteolytic digestion, of a sample that comprises an immunoglobulin polypeptide, at pH 8.8.
Claims12
A method for reducing the appearance of false positive bands in SDS-PAGE analysis of proteolytic digestion of a sample that comprises an immunoglobulin polypeptide;
- comprising incubating a mixture comprising the sample and the immunoglobulin polypeptide, for at least about 8 hours at a pH that causes fewer false positive bands in said SDS-PAGE analysis relative to pH 6.8, wherein said pH is 8.8, subjecting the mixture to electrophoresis on an SDS-PAGE gel and staining the gel with a protein indicator dye.
Dependencies are inferred from the claim text.