Back to results
US 8,569,002 B2Granted
Multiplexed luciferase reporter assay systems
AssigneeBoard of Regents, The University of Texas System
InventorsLawrence Lum, Ozlem Kulak
Life of this patent
Estimating the term…
Abstract
Inhibitors of luciferase enzymes are disclosed and find use in multiplexed assays using multiple luciferases and multiple inhibitors, in both in vitro and in vivo embodiments.
Claims18
A method of detecting a plurality of distinct luciferase molecules comprising:
- (a) providing a plurality of distinct luciferase molecules in combination with substrates therefor, wherein said plurality of distinct luciferase molecules includes at least renilla luciferase and firefly luciferase;
- (b) providing a plurality of distinct luciferase inhibitors, at least one of which selectively inhibits renilla luciferase and one of which selectively inhibits firefly luciferase molecules;
- (c) sequentially measuring chemiluminescence from said plurality of distinct luciferase molecules prior to and after combination of said plurality of distinct luciferase inhibitors with said plurality of distinct luciferase molecules,
- whereby the addition of said renilla luciferase inhibitor permits the detection of chemiluminescence from non-renilla luciferase molecules, and the addition of said firefly luciferase inhibitor permits the detection of chemiluminescence from non-firefly luciferase molecules.
1 more element
Dependencies are inferred from the claim text.