Detection of glycopeptides and glycoproteins for medical diagnostics

A method for detecting the presence, absence, progression, regression, or extent of a disease or disorder in a subject, comprising:

• a) obtaining two or more sample glycopeptides or glycoproteins from the subject;
• b) obtaining two or more reference glycopeptides or glycoproteins;
• c) comparing the sample glycopeptides or glycoproteins with the reference glycopeptides or glycoproteins using mass spectrometry;
• and
• d) detecting a difference in concentration between the sample glycopeptides or glycoproteins and the reference glycopeptides or glycoproteins;
• wherein a difference in concentration between the sample glycopeptides or glycoproteins and the reference glycopeptides or glycoproteins is indicative of the presence, absence, progression, regression, or extent of the disease or disorder.

The method of diagnosing a disease which comprises the steps of:

• a) providing a sample which comprises at least two glycopeptides or glycoproteins;
• b) providing a reference which comprises at least two glycopeptides or glycoproteins;
• and
• c) comparing the concentration of the sample glycopeptides or glycoproteins with the concentration of the reference glycopeptides or glycoproteins using mass spectrometry;
• wherein a difference in the concentration between the sample glycopeptides or glycoproteins and the reference glycopeptides or glycoproteins is an indicator of the disease.

A method of diagnosing a disease which comprises the steps of:

• a) providing a sample which comprises at least two glycopeptides or glycoproteins;
• b) providing a reference which comprises at least two glycopeptides or glycoproteins;
• and
• c) comparing the mass of the sample glycopeptides or glycoproteins with the mass of the reference glycopeptides or glycoproteins using mass spectrometry;
• wherein a difference in the mass between the sample glycopeptides or glycoproteins and the reference glycopeptides or glycoproteins is an indicator of the disease.