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US 8,568,976 B2Granted
Proinflammatory nucleic acids
AssigneeIsis Pharmaceuticals, Inc.
InventorJoseph Senn
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Abstract
The present invention provides compositions and methods for identifying and utilizing proinflammatory nucleic acids. In particular, this invention relates to compounds, particularly oligonucleotides, which exert their effect through triggering receptors expressed by myeloid cells (TREMs), specifically TREM 2.
Claims21
A method for identifying a proinflammatory oligonucleotide comprising:
- contacting a cell with a test oligonucleotide wherein:
- the cell functionally expresses a TREM-2 extracellular domain, an intracellular signaling domain, and a reporter construct comprising a cis-acting DNA consensus element sensitive to activation of the intracellular domain, and wherein the cis-acting DNA consensus element is functionally linked to a reporter gene coding sequence;
- and
- wherein the test oligonucleotide does not comprise a CpG motif, and wherein the oligonucleotide does not specifically hybridizes to a nucleic acid encoding TREM-2;
- assaying for activation of the reporter gene;
- and
- identifying test oligonucleotide as being proinflammatory if the reporter gene is activated.
4 more elements
Dependencies are inferred from the claim text.