US 8,541,652 B2 · Mutants of PHA synthase from Pseudomonas sp. 6-19 and method for preparing lactate homopolymer or copolymer using the same

Abstract

The present invention relates to polyhydroxyalkanoate synthase (PHA synthase) mutant originated from Pseudomonas sp. 6-19 (KCTC 11027BP) which can prepare lactate polymer and/or copolymer by using lactyl-CoA as a substrate. The present invention relates to a method for preparing lactate polymer and/or copolymer with the synthase mutant. The polyhydroxyalkanoate synthase mutants of the present invention originated from Pseudomonas sp. 6-19 can efficiently prepare lactate polymer and/or copolymer by using as a substrate lactyl-CoA which is difficult to be used as a substrate by conventional polyhydroxyalkanoate synthase.

Claims8

Dependent claims

A recombinant vector comprising:

a gene encoding propionyl-CoA transferase (pct), and
a gene encoding polyhydroxyalkanoate synthase mutant using lactyl-CoA as a substrate to produce lactate polymer or lactate copolymer,
wherein the polyhydroxyalkanoate synthase mutant has an amino acid sequence of SEQ. ID No:
10, wherein at least the glutamine at position 481 of the amino acid sequence of SEQ. ID No:
10 is mutated.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/312,676Filed 21 Nov 2007

Priority

KR 10-2006-011623423 Nov 2006

Published

US 2010/0050298 A125 Feb 2010

Granted

24 Sep 2013

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 9/1025
Transferases: acyltransferases
C12N 15/8243
With non-agronomic quality traits, e.g. for industrial processing; Value added, non-agronomic traits: involving biosynthetic or metabolic…
C12P 7/625
Carboxylic acid esters: polyesters of hydroxy carboxylic acids
C12N
Microorganisms or enzymes

Mutants of PHA synthase from Pseudomonas sp. 6-19 and method for preparing lactate homopolymer or copolymer using the same

Life of this patent

Mutants of PHA synthase from Pseudomonas sp. 6-19 and method for preparing lactate homopolymer or copolymer using the same

Life of this patent

Abstract

Claims8