Process for the functionalization of biological molecules
AssigneeCommissariat A L'energie Atomique Et Aux Energies Alternatives
InventorsAude Bernardin, David Bonnaffe, Isabelle Texier-Nogues
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Abstract
The present invention is targeted at a novel coupling method of 1,3-dipolar cycloaddition type which is of particular use for the functionalization of biological molecules, such as, for example, proteins, enzymes or nucleic acids, insofar as it can be carried out in an aqueous medium and at ambient temperature.
Claims17
A process of grafting at least one molecule of interest to an additional entity comprising at least the stages consisting in
- having available said molecule provided, at the surface, with at least one group G1 which is reactive according to a 1,3-dipolar cycloaddition reaction, and
- bringing together said molecule and said entity provided, at the surface, with at least one group G2 complementary to the group G1 from the viewpoint of said 1,3-dipolar cycloaddition, under conditions favorable to the cycloaddition of said groups G1 and G2,
- wherein said groups G1 and G2 are respectively a nitrone and a strained cycloalkynyl radical or vice versa.
A process of labeling a molecule of interest or of a substrate by a contrast agent comprising at least the stages consisting in:
- having available said molecule of interest or said substrate provided, at the surface, with at least one group G1 which is reactive according to a 1,3-dipolar cycloaddition reaction, and
- bringing together said molecule of interest or said substrate and said contrast agent provided, at the surface, with at least one group G2 complementary to the group G1 from the viewpoint of said 1,3-dipolar cycloaddition, under conditions favorable to the cycloaddition of said groups G1 and G2,
- said groups G1 and G2 respectively being a nitrone and a strained cycloalkynyl radical or vice versa.
Dependencies are inferred from the claim text.