Immunosuppressant drug extraction reagent for immunoassays
AssigneeAbbott Laboratories
InventorsFrank C. Grenier, Ryan F. Workman, Hina Syed, Salman Ali
Life of this patent
Estimating the term…
Abstract
An improved extractive reagent composition and method for extracting an immunosuppressant drug, such as sirolimus, tacrolimus or cyclosporine, from blood samples while yielding a test sample extract that has low vapor pressure and is compatible with immunoassay components. The inventive reagent composition comprises dimethyl sulfoxide (DMSO), at least one divalent metal salt and water. The sample extracts resulting from use of each of these combinations have low vapor pressure and are compatible with immunochemistry assays.
Claims13
An extractive reagent composition comprising dimethyl sulfoxide, a glycol, and at least one divalent metal salt of zinc and water, wherein the dimethyl sulfoxide is at least about 50% of the extractive reagent composition, the divalent metal salt of zinc is at a concentration of about 30 mM to about 75 mM in the extractive reagent composition, and the glycol is selected from the group consisting of ethylene glycol, propylene glycol, glycol analogs and mixtures thereof.
A test sample extract comprising dimethyl sulfoxide of at least about 33% by volume of the extract, at least one divalent metal salt of zinc, an antibody that binds to at least one immunosuppressant drug, and water.
An extractive reagent composition comprising dimethyl sulfoxide, a glycol, at least one divalent metal salt of zinc and water, wherein the dimethyl sulfoxide is at least about 50% by volume of the extractive reagent composition, the glycol is about 30%-33% by volume of the extractive reagent composition, and the divalent metal salt of zinc is at a concentration of about 5 mM to about 400 mM in the extractive reagent composition, wherein the glycol is selected from the group consisting of ethylene glycol, propylene glycol, glycol analogs and mixtures thereof.
A kit comprising:
- (a) at least one antibody that binds specifically to sirolimus;
- (b) an extractive reagent composition comprising dimethyl sulfoxide, a glycol, and at least one divalent metal salt of zinc and water, wherein the dimethyl sulfoxide is at least about 50% of the extractive reagent composition, the divalent metal salt of zinc is at a concentration of about 30 mM to about 75 mM in the extractive reagent composition, and the glycol is selected from the group consisting of ethylene glycol, propylene glycol, glycol analogs or mixtures thereof;
- and
- (c) a control composition comprising sirolimus or everolimus, wherein (a), (b) and (c) are provided in separate containers.
Dependencies are inferred from the claim text.