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US 8,541,549 B2Granted
Annexin-based apoptosis markers
AssigneeUniversity of Southern California
InventorRalf Langen
Life of this patent
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Abstract
The present invention describes an annexin derivative and a method of using the annexin derivative as a biosensor for real-time visualization of phosphatidylserine exposure, apoptosis, live-cell imaging and monitoring of cell health.
Claims17
An annexin derivative, comprising:
- the amino acid sequence of SEQ ID NO:
- 1 wherein residues 101 and 260 of SEQ ID NO:
- 1 have been substituted with cysteine residues, wherein said cysteine residues are conjugated to polarity sensitive fluorophore N,N′-Dimethyl-N-(iodoacetyl)-N′(7-nitrobenz-2-oxa-1,2-diazol-4-yl)ethylenediamine (IANBD) or 6-Bromoacetyl-2-dimethylaminonaphthalene (BADAN), and wherein residues 113 and 302 of SEQ ID NO:
- 1 are substituted with an amino acid other than cysteine or lysine.
1 more element
A kit comprising:
- an annexin derivative comprising the amino acid sequence of SEQ ID NO:1 wherein residues 101 and 260 of SEQ ID NO:1 have been substituted with cysteine residues and said cysteine residues are conjugated to a polarity sensitive fluorophore N,N′-Dimethyl-N-(iodoacetyl)-N′(7-nitrobenz-2-oxa-1,2-diazol-4-yl)ethylenediamine (IANBD) or 6-Bromoacetyl-2-dimethylaminonaphthalene (BADAN), and wherein residues 113 and 302 of said SEQ ID NO:1 are substituted with an amino acid other than cysteine or lysine;
- and instructions for using the annexin derivative to detect phosphatidylserine exposure.
Dependencies are inferred from the claim text.