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US 8,541,235 B2Granted
Modified reconstituted basement membrane composition for assay system
AssigneeCorning Incorporated
InventorsMin Wu, Frank J. Mannuzza
Life of this patent
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Abstract
A modified reconstituted extracellular matrix composition is provided herein. The composition includes an extracellular matrix and at least one exogenous component selected from heparin, fibronectin and laminin. The composition may have a basic pH. Additionally, provided herein is a cell culturing system including a substrate and a coating thereon of the composition to assess potential stimulators and/or inhibitors for their effects on various cell cultures while increasing the signal dynamic range.
Claims15
A method of preparing a cell-culturing system comprising:
- (a) providing an extracellular matrix composition comprising a growth factor reduced extracellular matrix extracted from Engelbreth-Holm-Swarm mouse sarcoma tumor;
- and at least one exogenous component selected from the group consisting of heparin, fibronectin and laminin;
- (b) applying the composition to a substrate;
- and
- (c) incubating the applied composition to allow polymerization thereof.
2 more elements
A method of preparing a cell-culturing system comprising:
- (a) providing an extracellular matrix composition comprising an extracellular matrix;
- and at least one exogenous component selected from the group consisting of heparin, fibronectin and laminin;
- (b) applying the composition to a substrate;
- and
- (c) incubating the applied composition to allow polymerization thereof,
- wherein the at least one exogenous component comprises heparin and fibronectin.
3 more elements
Dependencies are inferred from the claim text.