US 8,541,191 B2Granted

Hydrolases, nucleic acids encoding them and methods for biocatalytic synthesis of structured lipids

AssigneeBunge Oils, Inc.

InventorsChristopher L. G. Dayton, Tim Hitchman, Katie Kline, Jonathan Lyon, Mark A. Wall, Nelson R. Barton

Life of this patent

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Abstract

Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

Claims6

Dependent claims

A method of producing a diacylglyceride, the method comprising the steps of:

(a) providing an oil composition comprising a quantity of triacylglyceride,
(b) providing a polypeptide having a palmitase activity, said polypeptide being selected from the group consisting of isolated, synthetic and recombinant polypeptides having a palmitase activity and said polypeptide either
i. being encoded by
a nucleic acid comprising a nucleic acid sequence having at least 85% sequence identity to full length SEQ ID NO:1, and wherein the nucleic acid encodes at least one polypeptide having palmitase activity and the polypeptide having at least one amino acid residue change recited below in Table A, or
ii. having at least 85% sequence identity to full length SEQ ID NO:2, and having at least one amino acid residue change recited in Table A,
Table A
Amino
Original
New
Acid
Amino
Amino
Residue
Acid
Acid
27
G
Q
45
G
L
54
S
H
61
D
A
E
62
V
A
G
72
R
E
K
S
T
74
F
I
83
V
M
96
A
I
98
G
L
116
E
A
F
H
L
Q
Q
R, H140R
R
S
S
T
V
117
L
R, I161L
120
K
L
133
S
A
147
I
F
151
I
A
G
H
160
P
T
172
R
Q
212
S
R
213
K
T
214
T
V
N
R
Y
215
G
A
H
216
A
V
C
L
or
iii. having at least 85% sequence identity to full length of SEQ ID NO:2 and comprising at least one amino acid residue modification D61A;
D61E;
R72E;
R72K;
E116A;
E116Q;
E116R;
E116T;
E116V;
S133A;
I151G;
I151A;
V163R;
or D164R
(c) contacting said oil composition of step (a) with said polypeptide of step (b) under conditions sufficient for the polypeptide to hydrolyze a palmitase residue on the triacylglyceride to form a diacylglyceride.

121 more elements

Dependencies are inferred from the claim text.

Facts

Application: 13/083,480Filed 8 Apr 2011 · division
Priority: 29 Aug 2008
Published: US 2012/0276618 A11 Nov 2012
Granted: 24 Sep 2013
Term ends: Not estimatedThe source records don’t support an estimate.

Classification21 codes

C12P 7/6472
Glycerides: containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
A23D 7/04
Characterised by the production or working-up: working-up
A23D 9/04
Characterised by the production or working-up: working-up
C11B 3/003
Refining fats or fatty oils: by enzymes or microorganisms, living or dead

Family

Application12/202,204Parent of this division · filed 29 Aug 2008
US 2012/0276618 A1Pre-grant publication · published 1 Nov 2012
US 8,153,391 B2Parent of this division
US 2010/0055234 A1Published application
US 8,541,191 B2This patent

From the US filing history. Foreign family members aren’t listed.

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