Method for measuring the plasmine activity of microparticles present in a sample of blood or a blood component

Method for measuring the plasmin activity of microparticles, in a sample of biological fluid which is blood or a blood component, taken previously, comprising the following steps:

• in a first stage isolating said microparticles present in said sample according to a method comprising,
• centrifuging in a stage 1A in a volume between 500 μl and 5 ml, of a sample of biological fluid, taken previously, at a speed between 1,000 g and 2,000 g, for a time between 5 minutes and 20 minutes, at a temperature between 2 and 6° C. to obtain a first supernatant;
• centrifuging in a stage 1B the supernatant obtained in stage 1A at a speed between 10,000 g and 20,000 g, for a time between 1 minute and 5 minutes at a temperature between 2 and 6° C. to obtain a second supernatant;
• centrifuging in a stage 1C the second supernatant obtained in stage 1B at a speed between 15,000 g and 25,000 g, for a time between 45 and 120 minutes, at a temperature between 2 and 6° C., to obtain a pellet;
• in a stage 1D, taking up the pellet obtained in stage 1C in a volume of between 250 μl and 4 ml of phosphate buffered saline and centrifuging the mixture at a speed between 15,000 g and 25,000 g for between 45 and 120 minutes at a temperature between 2 and 6° C.;
• in a stage 1E, taking up the pellet obtained in stage 1D in a volume of between 250 μl and 4 ml of phosphate buffered saline and centrifuging the mixture at a speed between 15,000 g and 25,000 g for between 45 and 120 minutes at a temperature between 2 and 6° C.;
• in a stage 1F, taking up of the pellet obtained in stage 1E in a volume of between 20 μl and 500 μl of phosphate buffered saline;
• in a second stage, measuring the ability of said microparticles isolated in stage 1 to generate plasmin, and
• in a third stage, comparing the result of the measurement obtained in the second stage with the result of an identical measurement carried out under the same conditions on a control sample of identical biological fluid.