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US 8,541,179 B2Granted
Immunodetection assay for Mycobacterium tuberculosis complex
AssigneeBl Incorporated
InventorYasuharu Namba
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AssigneeBl Incorporated
InventorYasuharu Namba
Estimating the term…
The present invention provides a method for specifically detecting a Mycobacterium tuberculosis complex-specific secretory protein MPT64 antigen in a biological sample, whereby diagnosis of infection with Mycobacterium tuberculosis is carried out rapidly and safely with higher accuracy than before. An antibody that recognizes an epitope for MPB64 located in any one of amino acid sequences of SEQ ID NOS: 2 to 4, particularly a monoclonal antibody was obtained. Thus, an immunoassay using the antibody, particularly a sandwich immunoassay using first and second antibodies to MPB64, particularly an immunochromatographic assay and an immunochromatographic test strip are provided. A biological sample can be rapidly subjected to the immunoassay without culturing or after culturing for a time before Mycobacterium tuberculosis complex bacteria in the sample substantially start to grow. The biological sample may be pretreated by treatment for inactivation of Mycobacterium tuberculosis, or treatment by dispersion or solubilization.
A method for detecting Mycobacterium tuberculosis complex, which comprises an immunoassay using an antibody to a Mycobacterium tuberculosis complex-specific secretory protein MPB64, in which said antibody comprises a monoclonal antibody to an epitope of MPB64 located in any one of amino acid sequences of SEQ ID NOS:
A method for detecting Mycobacterium tuberculosis complex, which comprises a sandwich immunoassay using first and second antibodies to a Mycobacterium tuberculosis complex-specific secretory protein MPB64, in which at least one of the first and second antibodies comprises a monoclonal antibody to an epitope of MPB64 located in any one of amino acid sequences of SEQ ID NOS:
An immunochromatographic assay for detecting Mycobacterium tuberculosis complex, which comprises:
An immunochromatographic test strip for detecting a Mycobacterium tuberculosis complex, which at least comprises first and second antibodies to a Mycobacterium tuberculosis complex-specific secretory protein MPB64, and a membrane carrier, wherein the first antibody is previously immobilized in a predetermined position of the membrane carrier so as to form a capturing zone, the second antibody is labeled with a suitable labeling agent and is prepared at a position remote from the capturing zone for being chromatographically developed in the membrane carrier, wherein at least one of the first and second antibodies comprises a monoclonal antibody to an epitope of MPB64 located in any one of amino acid sequences of SEQ ID NOS:
A monoclonal antibody selected from the group consisting of:
Dependencies are inferred from the claim text.