Synergetic effect of the phytase combination on the hydrolysis of phytic acid

A method of hydrolysis of phytic acid (myo-inositol 1,2,3,4,5,6-hexakisphosphate) to inorganic monophosphates, to myo-inositols with a lower degree of phosphorylation and to free myo-inositol, comprising:

• hydrolyzing phytic acid with a first phytase and a second phytase,
• the first phytase exhibiting at least 95% identity with the 3-phytase of SEQ ID NO:
• 1 or exhibiting at least 95% identity with the 3-phytase of SEQ ID NO:
• 2, and
• the second phytase exhibiting at least 95% identity with the 3-phytase of SEQ ID NO:
• 3,
• wherein the first and second phytases have a synergistic hydrolysis effect on phytic acid in the absence of a phytase having a different stereospecificity.

A kit or assembly for feeding animals, comprising:

• the Schwanniomyces castellii 3-phytase of SEQ ID NO:
• 1 or the Debaryomyces castellii 3-phytase of SEQ ID NO:
• 2;
• and
• the Aspergillus niger 3-phytase of SEQ ID NO:
• 3,
• wherein the Schwanniomyces castellii 3-phytase of SEQ ID NO:
• 1 or the Debaryomyces castellii 3-phytase of SEQ ID NO:
• 2, in combination with the Aspergillus niger 3-phytase of SEQ ID NO:
• 3, have a synergistic hydrolysis effect on phytic acid in the absence of a phytase having a different stereospecificity.