Back to results
US 8,541,016 B2Granted
Cell-adhesive, enzymatically crosslinked flavonoid hydrogels and methods for making same
AssigneeAgency for Science, Technology and Research
InventorsMotoichi Kurisawa, Fan Lee, Joo Eun Chung, Pui Yik Chan
Life of this patent
Estimating the term…
Abstract
There are provided methods for producing a hydrogel that is capable of adhesion of cells and which comprises enzymatically cross-linked conjugates of a hydrogel forming agent and a flavonoid, formed from a reaction using peroxide and peroxidase. Hydrogels produced by such methods and methods of using the hydrogels are also provided.
Claims7
A method for producing a hydrogel that is capable of adhesion of cells and which comprises enzymatically cross-linked conjugates of a hydrogel forming agent and a flavonoid, the method comprising combining:
- (i) from about 0.1 mg/ml to about 500 mg/ml of conjugates of the hydrogel forming agent and the flavonoid;
- (ii) from about 0.001 mM to about 50 mM peroxide;
- and
- (iii) from about 0.001 units/ml to about 10 units/ml peroxidase;
- thereby producing the hydrogel.
2 more elements
Dependencies are inferred from the claim text.