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US 8,541,006 B2Granted
Methods and devices for the detection of biofilm
AssigneesUniversity of Maryland, Baltimore, The Arizona Board of Regents, a body corporate acting for and on behalf of Northern Arizona University
InventorsJeffrey G. Leid, Timothy L. Vail, Jennifer M. Kofonow, Mark E. Shirtliff, Rebecca A. Brady
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Abstract
The present invention provides methods and kits for biofilm detection.
Claims20
A method for detecting a probability for the presence of a Staphylococcus biofilm comprising:
- a) contacting a test sample with one or more detectably labeled proteins,
- wherein the one or more detectably labeled proteins are capable of binding antibodies present in the test sample, wherein the binding produces labeled antibodies;
- b) contacting the labeled antibodies to a substrate comprising one or more immobilized biofilm markers selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, SEQ ID NO:
- 43 and a polypeptide comprising one or more antigenic fragments thereof;
- and
- c) detecting binding of the labeled antibodies to the one or more immobilized biofilm markers, wherein binding indicates a probability for the presence of the Staphylococcus biofilm in the test sample.
3 more elements
A method for diagnosing a probability for the presence of a Staphylococcus biofilm related disease in a subject, comprising:
- a) contacting a test sample from the subject with one or more detectably labeled proteins, wherein the one or more detectably labeled proteins are capable of binding antibodies present in the test sample, wherein the binding produces labeled antibodies;
- b) contacting the labeled antibodies to a substrate comprising one or more immobilized biofilm markers selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, SEQ ID NO:
- 43 and a polypeptide comprising one or more antigenic fragments thereof;
- and
- c) detecting binding of the labeled antibodies to the one or more immobilized biofilm markers, wherein binding indicates a probability for the presence of a Staphylococcus biofilm related disease in the subject.
2 more elements
Biofilm detection substrates for detecting a probability for the presence of a Staphylococcus biofilm comprising:
- a) a test well comprising one or more detectably labeled proteins, wherein the one or more detectably labeled proteins are capable of binding to biofilm antibodies present in a test sample;
- and
- b) one or more immobilized Staphylococcus biofilm markers capable of binding to labeled antibodies selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:13, SEQ ID NO:
- 43 and a polypeptide comprising one or more antigenic fragments thereof.
2 more elements
Dependencies are inferred from the claim text.