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US 8,541,005 B2Granted
Cysteine-tagged streptococcal protein G variant
AssigneeKorea Research Institute of Bioscience and Biotechnology
InventorsBong Hyun Chung, Jeong Min Lee, Sun-Ok Jung, Yongwon Jung
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Abstract
The present invention relates to an N-terminal cysteine-tagged Streptococcal protein G variant. Since the variant binds in a directional manner to a surface of a biochip or a biosensor, the variant provides a biochip and a biosensor having an improved antibody-immobilizing capability, compared with an untagged protein G variant.
Claims15
An N-terminal cysteine-tagged Streptococcal protein G variant, represented by the following formula:
- Metx-(Cys)n-Ly-protein G-Qz
- wherein Met is methionine;
- L is a linker;
- protein G comprises two antibody-binding domains B1 and B2 of protein G obtained from Streptococcus;
- Q is a tag for protein purification;
- x, y and z are each 0 or 1;
- and n is a number from 1 to 10,
- wherein the antibody-binding domain B1 is represented by SEQ ID NO:
- 1, and the antibody-binding domain B2 is represented by SEQ ID NO:
- 2, and wherein the antibody-binding domain B1 has a deletion of 10 amino acid residues at its N-terminus.
7 more elements
Dependencies are inferred from the claim text.