Debriding composition from bromelain and methods of production thereof
AssigneeMediWound Ltd.
InventorsMarian Gorecki, Amir Toren
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Abstract
The present invention relates to a debriding composition obtained from bromelain and to methods of producing same. Particularly, the present invention relates to a debriding composition obtained from bromelain comprising proteolytic enzymes having molecular weights of about 23 kDa, being essentially devoid of bromelain inhibitors, and to pharmaceutical compositions comprising same. The debriding compositions and the pharmaceutical compositions comprising same are particularly useful in debriding eschar tissues and in wound healing.
Claims5
A debriding composition obtained from bromelain, the debriding composition comprising a plurality of different proteolytic enzymes having molecular weights of about 23 kDa as determined by size exclusion chromatography, wherein the plurality of different proteolytic enzymes comprises:
- (a) stem bromelain comprising SEQ ID. No. 1;
- (b) ananain comprising SEQ ID No. 2;
- (c) stem bromelain comprising SEQ ID No. 3;
- and
- (d) cysteine proteinase precursor comprising SEQ ID No. 4, said debriding composition comprising bromelain inhibitors in an amount of less than 10% w/w of protein content of the debriding composition, wherein the composition consists essentially of a single protein peak after elution from an HPLC size exclusion column TSK-Gel 3000SWXL, the single protein peak constituting proteins having molecular weights of about 23 kDa.
Dependencies are inferred from the claim text.