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AssigneeMascoma Corporation
InventorsElena E. Brevnova, Jim Flatt, Chhayal Gandhi, Vineet Rajgarhia, John McBride, Anne Warner
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The present invention provides for the isolation and characterization of the cbh1 gene from Schizochytrium aggregatum. In particular, the present invention provides for the nucleic acid and amino acid sequences of Schizochytrium aggregatum cbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression of Schizochytrium aggregatum Cbh1 in host cells, including yeast, e.g., Saccharomyces cerevisiae. Expression of Schizochytrium aggregatum Cbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression in Saccharomyces cerevisiae is in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novel Schizochytrium aggregatum Cbh1 gene can utilized in a consolidated bioprocessing system.
An isolated polypeptide comprising an amino acid sequence having at least 95% identity to amino acids 20 to 456 of SEQ ID NO:3, wherein said polypeptide has cellobiohydrolase activity.
An isolated polypeptide comprising an amino acid sequence having at least 95% identity to amino acids 457 to 546 of SEQ ID NO:
An isolated polypeptide comprising at least 50 contiguous amino acids within amino acids 20 to 456 of SEQ ID NO:3, wherein said polypeptide has cellobiohydrolase activity.
An isolated polypeptide comprising at least 50 contiguous amino acids within amino acids 457 to 546 of SEQ ID NO:
An isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide having at least 95% identity to amino acids 20 to 456 of SEQ ID NO:
An isolated polynucleotide comprising a nucleic acid sequence encoding amino acids 457 to 546 of SEQ ID NO:3 or amino acids 507 to 546 of SEQ ID NO:3, wherein said polypeptide has cellulose binding activity.
Dependencies are inferred from the claim text.