Polypeptides, systems, and methods useful for detecting glucose
AssigneeUniversity of Kentucky Research Foundation
InventorsSylvia Daunert, Kendrick Turner, Smita Joel, Laura Rowe
Life of this patent
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Abstract
The presently-disclosed subject matter provides biosensors for detecting molecules of interest. The biosensors include a polypeptide capable of selectively-binding glucose, wherein the polypeptide molecule is selected from: an unnatural analogue of wild type glucose binding protein; a fragment of wild type glucose binding protein; and an unnatural analogue fragment of wild type glucose binding protein.
Claims22
A biosensor for detecting a molecule of interest, comprising:
- an isolated polypeptide molecule capable of selectively-binding glucose, wherein the polypeptide molecule is selected from:
- an unnatural analogue of the amino acid sequence of SEQ ID NO:
- 2;
- and an unnatural analogue fragment of the amino acid sequence of SEQ ID NO:
- 2;
- and
- a label associated with the polypeptide molecule;
- wherein binding of glucose to the polypeptide molecule causes the label to generate a signal, such that the glucose can be detected.
An isolated polypeptide molecule capable of selectively-binding glucose, wherein the polypeptide molecule is selected from an unnatural analogue of the amino acid sequence of SEQ ID NO:
- 2;
- and an unnatural analogue fragment of the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.