Production of human late stage motor neuron progenitor cells
AssigneeCalifornia Stem Cell, Inc.
InventorAleksandra J. Poole
Life of this patent
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Abstract
Motor neuron progenitor (MNP) cells and populations of MNP cells, are provided, in particular, populations of human late stage MNP cells having a purity of greater than about 65% late stage MNP cells and high-purity populations of MNP cells having greater than 95% viable cells, as well as method of making and using the same, including deriving late stage MNP cells from pluripotent embryonic stem cells, producing high-purity populations of late stage MNP cells, producing populations of viable MNP cells, transporting viable MNP cells, and transplanting MNP cells.
Claims12
A method of preparing a population of viable human motor neuron progenitor cells comprising:
- (a) obtaining population of human late stage motor neuron progenitor cells having a purity of greater than about 65% late stage motor neuron progenitor cells, wherein the population is cultured on an adherent substrate and provides a layer of motor neuron progenitor cells;
- (b) dissociating the layer of motor neuron progenitor cells cultured on the adherent substrate to provide a cell suspension;
- (c) allowing the cell suspension to rest in a non-adherent environment for a period of time sufficient for floating microspheres comprising viable cells to form;
- (c) selecting the floating microspheres comprising viable cells;
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- (d) collecting the microspheres to provide a population of viable human motor neuron progenitor cells.
Dependencies are inferred from the claim text.