Systems and methods for ex-vivo organ care

An organ care system comprising:

• portable multiple use module including a portable chassis and a pump driver;
• and
• a single use disposable module including,
• a disposable chassis for interlocking the single use module with the multiple use module,
• a perfusion circuit for circulating perfusion fluid, said perfusion circuit including a pump interface having a housing in fluid tight interconnection with a surface that is movable with respect to the housing to translate pumping force from the pump driver to the perfusion fluid, and
• an organ chamber assembly mounted to the disposable chassis for containing a heart during perfusion, the organ chamber assembly including a first conduit for connecting the perfusion circuit to an aorta of the heart and a second conduit for connecting the perfusion circuit to a pulmonary vein of the heart,
• wherein the pump driver separably aligns with the pump interface upon interlocking the single use disposable module with the portable multiple use module and the pump interface mounts to the chassis of the single use module and includes features for interlocking with mating features of the pump driver for forming a separable fluid sealing interconnection with the pump driver
• the organ chamber assembly further comprising axially extending protuberances on the pump interface, the protuberances abutting a surface on the portable multiple use module to move the pump interface toward the pump driver on the multiple use module to form a separable fluid tight seal between the pump interface and the pump driver.