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US 8,465,958 B2Granted
Polypeptides having endoglucanase activity and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsAlfredo Lopez de Leon, Michael Rey
Life of this patent
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Abstract
The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims19
An isolated polynucleotide encoding a polypeptide having endoglucanase activity, selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity with the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polynucleotide encoding a polypeptide which hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO:
- 1, or (iii) the full-length complementary strand of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
- and
- (c) a polynucleotide encoding a polypeptide comprising a nucleotide sequence having at least 90% sequence identity with the mature polypeptide coding sequence of SEQ ID NO:
- 1.
5 more elements
Dependencies are inferred from the claim text.