Ethanol production in non-recombinant hosts
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsYoungnyun Kim, Keelnatham Shanmugam, Lonnie O. Ingram
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Abstract
Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.
Claims28
A method for producing ethanol from an oligosaccharide source comprising contacting the oligosaccharide with an isolated non-recombinant bacterium comprising an lpd gene having a mutation, wherein the mutation is one that corresponds to the substitution of the His residue at amino acid position 322 relative to an E. coli LPD sequence and the mutation renders the non-recombinant bacterium capable of producing ethanol as the primary fermentation product under anaerobic conditions, thereby producing ethanol from an oligosaccharide source.
A method for producing ethanol from an oligosaccharide source comprising contacting the oligosaccharide with an isolated non-recombinant bacterium comprising an E. coli lpd gene having a mutation selected from the group consisting of H322Y and E354K, wherein the mutation renders the non-recombinant bacterium capable of producing ethanol as the primary fermentation product under anaerobic conditions, thereby producing ethanol from an oligosaccharide source.
Dependencies are inferred from the claim text.