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US 8,465,952 B2Granted
Microorganism producing L-methionine precursor and the method of producing L-methionine precursor using the microorganism
AssigneeCj Cheiljedang Corporation
InventorsChul Ha Kim, So Young Kim, Young Uk Shin, Hye Won Um, Jin Sook Chang, Young Wook Cho, Han Jin Lee, In Kyung Heo, Chang II Seo, Kwang Ho Na
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Abstract
The present invention relates to a microorganism producing L-methionine precursor, O-acetylhomoserine, and a method of producing L-methionine precursor using the microorganism.
Claims16
A method for producing methionine, comprising,
- a) culturing a microorganism that produces and releases O-acetylhomoserine, wherein the microorganism is characterized by the following:
- i) a homoserine O-acetyltransferase activity (EC2.3.1.31) is introduced and enhanced by the integration of a gene from Deinococcus radiodurans that encodes homoserine O-acetyltransferase;
- and
- ii) an aspartokinase or homoserine dehydrogenase activity (EC2.7.2.4 or 1.1.1.3) is enhanced, but one or more of endogenous cystathionine gamma synthase, endogenous O-succinylhomoserine sulfhydrylase, and endogenous O-acetylhomoserine sulfhydrylase activities are reduced, inhibited or inactivated,
- b) producing methionine from the released O-acetylhomoserine.
2 more elements
Dependencies are inferred from the claim text.