Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
AssigneesNovozymes, Inc., Novozymes A/S
InventorsJunxin Duan, Lan Tang, Ye Liu, Wenping Wu, Jason Quinlan, Randall Kramer
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Abstract
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims19
A method of producing a mutant cell, comprising disrupting or deleting a nucleotide sequence encoding a polypeptide having cellulolytic enhancing activity in a parent Penicillium cell, which results in the mutant producing less of the polypeptide than the parent Penicillium cell, wherein the polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a polypeptide having at least 95% sequence identity to amino acids 22 to 332 of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by the polynucleotide having nucleotides 64 to 1018 of SEQ ID NO:
- 1;
- and (c) a fragment of a polypeptide of (a) or (b) that has cellulolytic enhancing activity.
A mutant cell, comprising a disruption or deletion of a nucleotide sequence encoding a polypeptide having cellulolytic enhancing activity in a parent Penicillium cell, wherein the mutant produces less of the polypeptide than the parent Penicillium cell, and wherein the polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to amino acids 22 to 332 of SEQ NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide comprising SEQ ID NO:
- 1;
- and (c) a fragment of SEQ ID NO:
- 2 having cellulolytic enhancing activity.
Dependencies are inferred from the claim text.