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AssigneeAB Enzymes Oy
InventorsCéline Cadot, Tina Ploss, Ruth Schwerdtfeger, Bruno Winter
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The present invention relates to a microbial expression system for the production of polypeptides based on the use of extrachromosomal DNA, whereby no antibiotic marker genes for the selection of the host cell but DNA sequences that encode glycerine-3-phosphate dehydrogenase are used, and, thus, the production of the desired polypeptide, e.g., xylanase, does not need the addition of antibiotics. The expression system is free from antibiotic-resistance genes. The invention further relates to a DNA sequence that encodes a polypeptide with glycerine-3-phosphate dehydrogenase activity as well as a polypeptide with glycerine-3-phosphate dehydrogenase activity.
An expression system for the production of one or more target polypeptide/target polypeptides, comprising a host cell in whose genome the DNA sequence that encodes glycerine-3-phosphate dehydrogenase is inactivated or partially or completely deleted and which is transformed by an extrachromosomal element that comprises a DNA sequence that encodes the target polypeptide(s) and glycerine-3-phosphate dehydrogenase, whereby not only the host cell genome but also the extrachromosomal element do not carry an antibiotic-resistance gene.
Dependencies are inferred from the claim text.