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US 8,465,945 B2Granted
Method for production and use of mite group 1 proteins
AssigneeMerck Patent GmbH
InventorsElaine A. Best, Martin J. McDermott
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AssigneeMerck Patent GmbH
InventorsElaine A. Best, Martin J. McDermott
Estimating the term…
The present invention includes a method to produce a recombinant mite Group 1 protein in a methyltrophic yeast or an Escherichia coli microorganism. The present invention also relates to a recombinant mite Group 1 protein obtained by such a method, such a recombinant protein being able to selectively bind IgE or cause proliferation of a T cell that proliferate in response to a native mite Group 1 protein. Also included in the present invention is the use of such a recombinant mite Group 1 protein to detect mite allergy or to reduce an allergic response to a mite Group 1 protein. The present invention also includes novel mite Group 1 nucleic acid molecules, proteins, recombinant molecules, and recombinant cells, as well as uses thereof.
A method to produce a recombinant protein comprising a recombinant mite Group 1 protein, comprising the steps of:
A methyltrophic yeast microorganism comprising a nucleic acid molecule encoding a mite Group 1 protein wherein, said nucleic acid molecule consists of the nucleic acid sequence of SEQ ID NO:10, and said recombinant mite Group 1 protein is a Dermatophagoides pteronyssinus, recombinant mite Group 1 protein (Der p 1), said nucleic acid molecule being operatively linked to a transcription control sequence and wherein sad nucleic acid molecule is optionally fused to a signal sequence.
Dependencies are inferred from the claim text.