US 8,465,933 B2Granted

Method for screening human ABO blood group-binding lactobacilli

AssigneeMeiji Co., Ltd.

InventorsTadao Saito, Yasushi Kawai, Hideaki Uchida, Katsunori Kimura, Kakuhei Isawa, Keisuke Furuichi

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Abstract

Lactobacillus screening methods were carried out using surface plasmon resonance spectrums and human intestinal mucin and blood group antigens as probes. A trial to set selection criteria in the above-mentioned methods of screening for lactobacilli was made to adapt the methods to mass screening, and it was discovered that lactobacilli compatible with ABO blood groups can be screened by setting 100 RU as a criterion for judging bacterial binding under certain conditions. Using 238 lactobacillus strains, the above-mentioned screening methods and tests to judge their compatibility for the use of yogurt production were carried out, to at long last specifically discover bacillus strains compatible with blood groups A, B, and O.

Claims9

Dependent claims

A method of screening for a human blood group A-compatible lactobacillus, comprising:

(a) providing a candidate lactobacillus;
(b) determining ability of the candidate lactobacillus to recognize a human blood group A antigen, a human blood group B antigen, and a human blood group H antigen, wherein the recognizing ability is determined by measuring response units (RU) of surface plasmon resonance spectrum, wherein the human blood group A antigen comprises [GalNAcα1-3(Fucα1-2)Gal-];
and
(c) selecting the candidate lactobacillus as a human blood group A-compatible lactobacillus if:
(i) blood group A antigen-recognizing ability is 2,000 RU or higher;
(ii) blood group B antigen-recognizing ability is 100 RU or lower;
(iii) blood group H antigen-recognizing ability is 100 RU or lower;
(iv) ratio of the blood group A antigen-recognizing ability to the blood group B antigen-recognizing ability is 70 or higher;
(v) ratio of the blood group A antigen-recognizing ability to the blood group H antigen-recognizing ability is 100 or higher;
(vi) A-HCM-recognizing ability is 100 RU or higher;
and
(vii) O-HCM-recognizing ability is 100 RU or lower.

9 more elements

Dependencies are inferred from the claim text.

Facts

Application: 12/987,518Filed 10 Jan 2011 · division
Priority: JP 2004-3491351 Dec 2004
Published: US 2011/0104721 A15 May 2011
Granted: 18 Jun 2013
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

A23C 9/1234
Using only microorganisms of the genus lactobacteriaceae; Yoghurt: characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus,…
C12Q 1/04
Involving viable microorganisms: determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides;…
G01N 33/54373
Apparatus specially adapted for solid-phase testing: involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
G01N 33/56911
For microorganisms, e.g. protozoa, bacteria, viruses: bacteria

Family

PCT/JP2005/022096Parent of this division · filed 1 Dec 2005
US 2011/0104721 A1Pre-grant publication · published 5 May 2011
Application11/720,462Parent of this division
US 7,897,374 B2Parent of this division
US 2008/0160565 A1Published application
US 8,465,933 B2This patent

From the US filing history. Foreign family members aren’t listed.

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