Anti-cobra toxin antibody fragments and method of producing a VHH library
AssigneeUniversity of Guelph
InventorsJ. Christopher Hall, Gabrielle Richard, Michael D. McLean
Life of this patent
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Abstract
A method of constructing a VHH library from an immunized camelid, using whole venom or an extract thereof There is also provided VHH antibody fragments isolated from a library produced in hyperimmunized llama These VHH antibody fragments were sequenced, and specifically bind α-cobratoxin.
Claims15
An isolated VHH antibody fragment obtained from a VHH library prepared by a method comprising:
- (1) immunizing a camelid with whole venom or an extract thereof;
- (2) isolating nucleic acid sequences encoding the variable heavy fragment from the immunized camelid;
- and
- (3) transforming a suitable host with the nucleic acid sequences to prepare a recombinant VHH library comprising VHH antibody fragments, wherein the VHH antibody fragments comprise a heavy chain complementarity determining region 1 (CDR1) comprising the amino acid sequence of SEQ ID NO:5;
- a heavy chain complementarity determining region 2 (CDR2) comprising the amino acid sequence of SEQ ID NO:9;
- and a heavy chain complementarity determining region 3 (CDR3) comprising the amino acid sequence of SEQ ID NO:11.
An isolated VHH antibody fragment comprising a heavy chain complementarity determining region 1 (CDR1) comprising the amino acid sequence of SEQ ID NO:5;
- a heavy chain complementarity determining region 2 (CDR2) comprising the amino acid sequence of SEQ ID NO:9;
- and a heavy chain complementarity determining region 3 (CDR3) comprising the amino acid sequence of SEQ ID NO:11.
An isolated VHH antibody fragment comprising the amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.