Methods for increasing protein polyethylene glycol (PEG) conjugation
AssigneeAntiCancer, Inc.
InventorsShukuan Li, Zhijian Yang, Xinghua Sun, Yuying Tan, Shigeo Yagi
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Abstract
The present invention relates to highly conjugated proteins and methods for making such proteins. In particular, the present invention relates to methods for linking additional sites to a protein for conjugation with activated polyethylene glycol (PEG) linkers, without denaturing the protein. The invention also relates to highly conjugated proteins with decreased immunogenicity and increased circulating half-life.
Claims12
A modified methioninase protein wherein said modification comprises at least one first polyethylene glycol (PEG) polymer coupled to at least one amino group of said methioninase and at least one second PEG polymer coupled to a carboxyl group of said methioninase through a polyamine linker, wherein one amino group of said polyamine linker forms an amide with said carboxyl group of the methioninase and at least one additional amino group is coupled to said second PEG polymer.
Dependencies are inferred from the claim text.