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AssigneeJCR Pharmaceuticals Co., Ltd.
InventorsHiroyuki Shirono, Youngjin Byun, Shoichiro Kamei, Kiwamu Imagawa
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Disclosed are a method for producing a frozen pharmaceutical composition prepared from cultured human mesenchymal stem cells and a pharmaceutical composition containing human mesenchymal stem cells. This method is a method for producing a frozen pharmaceutical composition containing human mesenchymal stem cells, which comprises the following steps in this order: (a) adding trypsin to human mesenchymal stem cells in a culture vessel to detach the cells from the surface of the culture vessel; (b) adding a bicarbonate Ringer's solution containing human serum albumin to the detached cells to terminate the reaction with trypsin, and washing the cells with the bicarbonate Ringer's solution containing human serum albumin; (c) suspending the cells in a bicarbonate Ringer's solution containing human serum albumin and dimethyl sulfoxide; (d) putting the resulting suspension in a container which allows freezing of what is contained therein, and sealing the container; and (e) freezing the suspension put in the container.
A pharmaceutical composition which comprises human mesenchymal stem cells in a bicarbonate Ringer's solution containing human serum albumin, dimethyl sulfoxide, 120-150 mEq/L of sodium ion, 3.6-4.4 mEq/L of potassium ion, 2.7-3.3 mEq/L of calcium ion, 0.9-1.1 mEq/L of magnesium ion, 100-125 mEq/L of chloride ion, 22-28 mEq/L of bicarbonate ion, and 4.5-5.5 mEq/L of citrate ion.
A method for preparation of human mesenchymal stem cells free of the medium used for their culture comprising collecting the human mesenchymal stem cells from a culture vessel in which they were allowed to proliferate, which method further comprises the steps:
Dependencies are inferred from the claim text.