Endothelial cells expressing adenovirus E4ORF1 and methods of use thereof
AssigneesCornell Research Foundation, Inc., Sloan-Kettering Institute for Cancer Research
InventorsShahin Rafii, Fan Zhang, Marco Seandel
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Abstract
The present invention relates to adenovirus E4ORF 1 gene and to endothelial cells engineered to express the E40RF 1 gene. The present invention also relates to uses of the E40RF 1 gene, and cells expressing the E40RF1 gene, and to compositions comprising the E4ORF 1 gene, or comprising cells expressing the E4ORF 1 gene.
Claims23
A method for maintaining or expanding endothelial cells in culture, comprising:
- (a) introducing a nucleic acid sequence encoding adenovirus E4ORF1 under the control of a promoter into isolated endothelial cells, wherein the nucleic acid sequence does not encode adenovirus E4ORF2, E4ORF3, E4ORF4, E4ORF5, or E4ORF6, such that the endothelial cells express E4ORF1 to a level sufficient to maintain or expand the isolated endothelial cells in culture, and
- (b) culturing the isolated endothelial cells expressing E4ORF1.
A population of isolated endothelial cells expressing adenovirus E4ORF1 but not expressing adenovirus E4ORF2, E4ORF3, E4ORF4, E4ORF5, or E4ORF6.
A method of culturing primary cancer cells, stem cells or progenitor cells, comprising:
- (a) obtaining or generating a population of isolated E4ORF1-expressing human umbilical vein endothelial cells, wherein the E4ORF1-expressing human umbilical vein endothelial cells do not express E4ORF2, E4ORF3, E4ORF4, E4ORF5, or E4ORF6, and
- (b) culturing the E4ORF1-expressing human umbilical vein endothelial cells in the same culture vessel with primary cancer cells, stem cells or progenitor cells.
A method of culturing primary cancer cells, stem cells or progenitor cells, comprising
- (a) obtaining or generating a population of isolated E4ORF1-expressing human umbilical vein endothelial cells, wherein the E4ORF1-expressing human umbilical vein endothelial cells do not express E4ORF2, E4ORF3, E4ORF4, E4ORF5, or E4ORF6,
- (b) culturing the E4ORF 1-expressing human umbilical vein endothelial cells in a culture vessel,
- (c) collecting conditioned medium from the culture vessel, and
- (d) adding the conditioned medium to a culture of primary cancer cells, stem cells or progenitor cells.
A method for maintaining or expanding endothelial cells in culture, the method comprising:
- (a) obtaining a population of isolated endothelial cells expressing adenovirus E4ORF1 to a level sufficient to maintain or expand the isolated endothelial cells in culture, but not expressing adenovirus E4ORF2, E4ORF3, E4ORF4, E4ORF5, or E4ORF6, and
- (b) culturing the population of isolated endothelial cells.
Dependencies are inferred from the claim text.