Biomarkers for the diagnosis of ALS
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorRobert P. Bowser
Life of this patent
Estimating the term…
Abstract
Methods for determining the onset of ALS in a subject are provided. One method includes analyzing a sample obtained from the subject for the presence or amount of one or more biomarkers indicative of ALS. In a preferred embodiment, the biomarkers are one or more of the following: C-reactive protein (CRP), cystatin c, plasminogen, complement C3, CysGly-transthyretin, and phosphorylated neurofilament heavy chain (pNFH). The sample is typically cerebral spinal fluid (CSF). The levels or concentrations of the biomarkers can be used to determine the onset of ALS, monitor the progression of ALS, or monitor the progression of a treatment for ALS.
Claims21
A method for selecting a subject for treatment of amyotrophic lateral sclerosis (ALS) comprising
- measuring the concentration of phosphorylated neurofilament heavy chain (pNFH) in a blood or cerebral spinal fluid sample obtained from the subject;
- measuring the concentrations of complement C3 (C3) in the sample;
- determining a ratio of pNFH to C3;
- and
- selecting the subject for treatment if the ratio of pNFH/C3 is greater than 0.000125 and the concentration of pNFH is greater than 0.635 ng/ml,
- wherein the concentrations of pNFH and C3 are measured by immunoassay or mass spectrometric measures.
A method for assisting in diagnosing amyotrophic lateral sclerosis (ALS) in a subject comprising:
- measuring the concentration of phosphorylated neurofilament heavy chain (pNFH) in a blood or cerebral spinal fluid sample obtained from the subject;
- measuring the concentration of complement C3 (C3) in the sample;
- measuring the concentration of C Reactive Protein (CRP) in the sample;
- determining a ratio of pNFH to C3;
- determining a ratio of pNFH to CRP;
- wherein a concentration of pNFH greater than 0.635 ng/ml and a ratio of pNFH/C3 greater than 0.000125 and a ratio of pNFH/CRP greater than 0.3755 is indicative of ALS,
- wherein the concentrations of pNFH, C3, and CRP are measured by immunoassay or mass spectrometric measures.
A method for selecting a subject for treatment of amyotrophic lateral sclerosis (ALS) comprising:
- measuring the concentration of phosphorylated neurofilament heavy chain (pNFH) in a blood or cerebral spinal fluid sample obtained from the subject;
- measuring the concentration of complement C3 (C3) in the sample
- measuring the concentration of C Reactive Protein (CRP) in the sample,
- determining a ratio of pNFH to C3;
- determining a ratio of pNFH to CRP;
- selecting the subject for treatment when the concentration of pNFH is greater than 0.635 ng/ml and the ratio pNFH/C3 is greater than 0.000125 and the ratio of pNFH/CRP is greater than 0.3755 is indicative of ALS,
- wherein the concentrations of pNFH, C3, and CRP are measured by immunoassay or mass spectrometric measures.
A method for assisting in diagnosing amyotrophic lateral sclerosis (ALS) in a subject comprising:
- measuring the concentrations of phosphorylated neurofilament heavy chain (pNFH) in a blood or cerebral spinal fluid sample obtained from the subject;
- measuring the concentration of complement C3 (C3) in the sample;
- determining a ratio of pNFH to C3,
- wherein a ratio greater than 0.000125 is indicative of ALS,
- wherein the concentrations of pNFH and C3 are measured by immunoassay or mass spectrometric measures.
A method for assessing the efficacy of a treatment for amyotrophic lateral sclerosis (ALS) comprising
- administering a drug to a subject having ALS,
- measuring the concentrations of pNFH and complement C3 (C3) in a blood or cerebrospinal fluid sample obtained from the subject before and after administration of the drug;
- wherein the treatment is effective if the ratio of pNFH to C3 decreases after administration of the drug relative to the ratio of pNFH to C3 prior to administration of the drug,
- wherein the concentrations of pNFH and C3 are measured by immunoassay or mass spectrometric measures.
A method for assisting in diagnosing amyotrophic lateral sclerosis (ALS) in a subject comprising:
- measuring the concentration of phosphorylated neurofilament heavy chain (pNFH) in a blood or cerebral spinal fluid sample obtained from the subject;
- measuring the concentration of complement C3 (C3) in the sample;
- measuring the concentration of C Reactive Protein (CRP) in the sample;
- determining a ratio of pNFH to C3;
- determining a ratio of pNFH to CRP;
- wherein a concentration of pNFH greater than 0.635 ng/ml, and a ratio of pNFH/C3 greater than 0.000125, or a ratio of pNFH/CRP greater than 0.3755 is indicative of ALS, wherein the concentrations of pNFH, C3, and CRP are measured by immunoassay or mass spectrometric measures.
Dependencies are inferred from the claim text.