Non-adhesive elastic gelatin matrices
AssigneeCovalon Technologies Inc.
InventorsValerio DiTizio, Frank DiCosmo, Yuehua Xiao
Life of this patent
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Abstract
The present invention is a substantially non-adhesive elastic gelatin matrix. The matrix is both non-adhesive to wounds, tissues and organs and is also elastic such that it is flexible. The matrix is a lyophilized mixture of protein(s), polymer(s), cross-linking agent(s) and optional plasticizer(s). The invention also provides methods for making the non-adhesive elastic gelatin matrix.
Claims16
An elastic non-adhesive protein matrix comprising:
- (a) about 50-90% by wgt of one or more proteins selected from the group consisting of collagen, and denatured collagen and mixtures thereof;
- (b) about 10-40% by wgt of one or more biocompatible polymers selected from the group consisting of polyetheylene glycol, poly-L-lysine, alginate, chitosan, hyaluronic acid, and chondroitin sulfate and mixtures thereof;
- and
- (c) about 0.5-5% by wgt of diglycidyl ether;
- wherein the matrix is lyophilized after addition of (c) to result in a matrix that is non-adherent to wounds tissues, and organs and can be removed after 24 hours.
A method for making a non-adhesive elastic protein matrix, the method comprising, in order, the steps of:
- (a) mixing collagen, denatured collagen, or a combination thereof in a solution and heating said solution;
- (b) adding polyethylene glycol, poly-L-lysine, alginate, chitosan, hyaluronic acid, or chondroitin sulfate or any combination thereof to the solution of (a);
- (c) adding 1-[3-(dimethylamino)propyl]-3-ethyl carbodiimide (EDC), N-hydroxysuccinimide (NHS), formaldehyde, glutaraldehyde, a polyaziridine, or a diglycidyl ether or any combination thereof to the solution of (b) under conditions that induce protein cross-linking while maintaining a solution;
- (d) mixing (c) until a pourable gel is formed;
- (e) cooling the gel of (d);
- and
- (f) lyophilizing the cooled gel of (e) to form the non-adhesive elastic protein matrix;
- wherein the non-adhesive elastic protein matrix comprises:
- about 50-90% by wgt collagen or denatured collagen or a combination thereof;
- about 10-40% by wgt polyetheylene glycol, poly-L-lysine, alginate, chitosan, hyaluronic acid, or chondroitin sulfate or any combination thereof;
- about 0.5-5% by wgt of 1-[3-(dimethylamino)propyl]-3-ethyl carbodiimide (EDC), N-hydroxysuccinimide (NHS), formaldehyde, glutaraldehyde, a polyaziridine, or a diglycidyl ether or any combination thereof;
- and
- wherein the matrix is non-adherent to wounds, tissues and organs and can be removed after 24 hours.
A method for making a non-adhesive elastic protein matrix, the method comprising, in order, the steps of:
- (a) mixing 0.5-30% by wgt collagen or denatured collagen;
- up to 10% by wgt of one or more biocompatible polymers;
- 0.1-2% by wgt of one or more cross-linking agents;
- about 50% to about 99% by weight solvent;
- and optionally up to 5% by weight plasticizer in a solution until a pourable gel is formed;
- (b) cooling the gel of (a);
- and
- (c) lyophilizing the cooled gel of (b) to form the non-adhesive elastic protein matrix that is removable after 24 hours.
A method for making a non-adhesive elastic protein matrix, the method comprising, in order, the steps of:
- (a) mixing collagen or denatured collagen or a combination thereof in a solution and heating said solution;
- (b) adding polyethylene glycol, poly-L-lysine, alginate, chitosan, hyaluronic acid, or chondroitin sulfate or any combination thereof to the solution of (a);
- (c) adding one or more cross-linking agents selected from the group consisting of 1-[3-(dimethylamino)propyl]-3-ethyl carbodiimide (EDC), N-hydroxysuccinimide (NHS), formaldehyde, glutaraldehyde, a polyaziridine, and a diglycidyl ether or any combination thereof to the solution of (b);
- (d) cooling the solution of (c);
- and
- (e) lyophilizing the cooled solution of (d) to form the non-adhesive elastic protein matrix that can be removed after 24 hours.
Dependencies are inferred from the claim text.