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US 8,273,528 B2Granted
Methods and compositions for determining methylation profiles
AssigneesCold Spring Harbor Laboratory, Washington University
InventorsRobert Martienssen, Eric J. Richards, Zachary Lippmann, Vincent Colot
Life of this patent
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Abstract
Methods and compositions for determining the methylation profile of individuals and using the profiles to identify clones with desired traits.
Claims14
A method for determining the relative amount of methylated or unmethylated DNA comprising a nucleic acid sequence of interest, wherein the DNA is from a cell, tissue or organism, the method comprising the steps of:
- a. providing a population of randomly cleaved or sheared DNA fragments from the cell, tissue, or organism, wherein the DNA comprises methylated and unmethylated fragments;
- b. depleting methylated or unmethylated DNA from the population;
- and
- c. quantifying the amount of the nucleic acid sequence of interest in the methylated DNA in the depleted population or the amount of the nucleic acid sequence of interest in the unmethylated DNA in the depleted population relative to the amount of the sequence in total genomic DNA.
1 more element
Dependencies are inferred from the claim text.